Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 14

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: Sap ^−/− T cells fail to stably interact with B cells when examined together with Sap ^+/+T cells in the same LN in vivo

Sap ^+/+ OT-2 T cells (red), Sap ^−/− OT-2 T cells (green), and MD4 B cells (blue) were cotransferred into mice that had previously been immunized with HEL-OVA conjugate antigen. Intravital imaging of the draining LNs was performed 24 to 36 h after cell transfer. The circle at the beginning of this video highlights a three-cell cluster in which an MD4 B cell is engaged by both a Sap ^+/+ and a Sap ^−/− OT-2 T cell. The Sap ^−/− T cell then disengages quickly, while the Sap ^+/+ T cell remains in conjugation with the B cell for a much longer period of time.

Original publication: Qi H, Cannons JL, Klauschen F, Schwartzberg PL, Germain RN. 2008. SAP-controlled T-B cell interactions underlie germinal centre formation. Nature 455:764–69